GRE-ACF-SP23-16- Hemiptera

April 24, 2023

Sample information
Picture
Photos by: Chelsea G
Location
Collection date 01/25/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Date of collection3: 1/25/2023

Time of collection3: 1:12 PM

Weather conditions3: Cool, gloomy day- right before rain

  • 68° F- scattered clouds

Location of collection3: Outside of Science Center and Greenhouse on Georgia Southern Armstrong Campus

Environment of collection (habitats)5: Bushes, Trees, dirt, under tree branches on the ground, open area of grass

Method of collection5: 3 methods of collection

  • Sweep net
  • Canvas and beat stick
  • Digging in dirt
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

When loading my wells, the samples disappeared while waiting. They did not show up during the process either, even though i loaded to wells correctly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
Report Inappropriate Post