GRE-ACF-SP23#4

April 19, 2023

Sample information
Picture
Location No map location data available.
Collection date 01/25/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught in bushes and trees with an arial net then by hand.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I believe that the controls were inputted correctly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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