HM02 – Seven Spotted Lady Beetle

May 12, 2023

Sample information
Picture
Location
Collection date 04/06/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations
  • Black and white stripped body
  • 7 spots
  • White eyes and nostrils
  • Red with black spots casing
  • Black/Transparent long wings
  • 6 legs
Putative identification Arthropoda Insecta Coleoptera Coccinellidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes
  • Lane 1 : DNA Ladder
  • Lane 2 : AD83/Spider
  • Lane 3 : HM02/Ladybug
  • Lane 4 : (+) Arthropod Control
  • Lane 5 : (-) Arthropod Control
  • Lane 6 : (+) DNA Control
  • Lane 7 : Water
  • Lane 8 : N/A

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I am half confident because our lady bug did not show a band for our Wolbachia gel; however, our + Wolbachia control did not have a band while our – Wolbachia control did.  This leads me to believe there is a source of error in either our DNA extraction or PCR protocol.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coccinellidae was found to be negative for Wolbachia.
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