AD83 Spider Arachnida

May 12, 2023

Sample information
Picture
Photos by: Aji D., Noreen G.
Location
Collection date 04/06/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

Had dark brown spots on translucent yellow tan legs, dark brown abdomen
Putative identification Arthropoda Arachnida

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes
  • Lane 1 : DNA Ladder
  • Lane 2 : AD83/Spider
  • Lane 3 : HM02/Ladybug
  • Lane 4 : (+) Arthropod Control
  • Lane 5 : (-) Arthropod Control
  • Lane 6 : (+) DNA Control
  • Lane 7 : Water
  • Lane 8 : N/A

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Based on our results, I am partially confident that the results we received are accurate. According to our gel runs, the spider arthropod sample has presence of wolbachia. However, our wolbachia positive control did not have a band (or was rather faint) while our negative control did. It is plausible that a source of error may have occured in protocol such as switching the negative and positive control tubes.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arachnida was found to be postive for Wolbachia.
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