Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/12/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Antioch High School |
| Observations | The Sweat Bee was located outside, sitting on the ledge of a picnic table. I used my collection tube to slowly trap the bug inside. After I caught the Bee I placed it Pitri Disk, to further investigate it. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | Wizard SV (Promega) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We froze our samples halfway through extraction. During extraction I dropped one of the samples on the floor. The sample did not seem to spill out. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | I am not super confident in my results because, some of the bands and controls didn’t make sense or did not add up. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
DKH10
DKH 9
DKH8
DKH 7
DKH 6