Sample information
Picture	Photos by: Jennifer V
Location
Collection date	09/11/2023
Captive / Cultivated?	Wild-caught
Group	Antioch High School
Observations
Putative identification	Arthropoda Hexapoda Insecta Diptera Calliphoridae Phormia Phormia regina
Methods
Extraction kit	Wizard SV (Promega)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TBE
DNA stain	SYBR Safe
Gel images
Protocol notes	Samples were frozen due to time running out
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Phormia regina was found to be negative for Wolbachia.