Phonmia Regina (black blow fly)

Sample information

Photos by: Jennifer V
Collection date 09/11/2023
Captive / Cultivated? Wild-caught
Group Antioch High School
Putative identification Arthropoda Hexapoda Insecta Diptera Calliphoridae Phormia Phormia regina


Extraction kit Wizard SV (Promega)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Samples were frozen due to time running out


Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Phormia regina was found to be negative for Wolbachia.
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