Phonmia Regina (black blow fly)

December 7, 2023

Sample information
Picture
Photos by: Jennifer V
Location
Collection date 09/11/2023
Captive / Cultivated? Wild-caught
Group Antioch High School
Observations
Putative identification Arthropoda Hexapoda Insecta Diptera Calliphoridae Phormia Phormia regina

Methods
Extraction kit Wizard SV (Promega)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Samples were frozen due to time running out

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Phormia regina was found to be negative for Wolbachia.
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