Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/15/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Dark green coloring on the top of the body yellow covering the bottom. Two antennae, long and black. Long bendable legs with little spikes toward the |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The used pippet tip went into liquid but the used tip was not used, just submerged in the liquid. It was used twice with non-DNA sample liquids. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | No steps were messed up to a degree that would possibly contaminate the DNA |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Orthoptera was found to be negative for Wolbachia. |
Water Boatman