Horned Passalus Beetle (Odontotaenius disjunctus)

November 20, 2023

Sample information
Picture
Photos by: Elijah S.
Location
Collection date 09/18/2023
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Black. Large abdomen with parallel grooves running down the length. Short mandibles. Short orange fur on underside.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA EXTRACTION

It was a fun time slicing up this bug!

MISTAKES

We didn’t make any mistakes during the arthropod electrophoresis, but something went wrong for our wolbachia electrophoresis the first time we did it. Luckily, we repeated the experiment and all looks to have gone well.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

DNA extraction

Some of the DNA for this sample clogged up the filter, making it hard to wash.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
ACTTGGAACTTCATTAAGACTATTAATTCGGATAGAATTAGGAACCCCTGGTTCATTAATTGGGAACGACCAAATCTATA ACTCTATCGTCACAGCTCATGCTTTTATCATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGA TTAGTTCCTCTAATACTAGGAGCTCCAGATATAGCTTTCCCTCGTATAAATAATATAAGATTTTGATTACTTCCTCCTTC TATTTCATTACTATTAGCTAGAAGATTTATTGAGTCAGGGGCGGGAACAGGATGAACAGTTTACCCTCCTCTTTCCAGTA ATATTGCCCACAGTGGAGCCTCAGTAGATTTAACAATTTTTAGCCTTCATTTGGCAGGAATCTCTTCAATTTTGGGGGCA GTTAATTTTATCTCTTCAATTATAAATATACGAACCCCTGGAATAACAATAGAAAAAATACCTTTATTTGCTTGATCTGT AGGAATTACTGCCGTTCTATTACTTCTTTCTCTCCCAGTATTAGCAGGAGCAATTACAATACTTTTAACCGATCGAAATT TAAATACCTCATTTTTTGACCCAACCGGAnGAGGAGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
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