|Captive / Cultivated?||Wild-caught|
|Group||Edmund Burke School|
Black. Large abdomen with parallel grooves running down the length. Short mandibles. Short orange fur on underside.
|Putative identification||Arthropoda Hexapoda Insecta|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Partial abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||MiniOne|
It was a fun time slicing up this bug!
We didn’t make any mistakes during the arthropod electrophoresis, but something went wrong for our wolbachia electrophoresis the first time we did it. Luckily, we repeated the experiment and all looks to have gone well.
|Explanation of confidence level||
Some of the DNA for this sample clogged up the filter, making it hard to wash.
|Wolbachia 16S sequence||
|Arthropod COI sequence|| Download FASTA
ACTTGGAACTTCATTAAGACTATTAATTCGGATAGAATTAGGAACCCCTGGTTCATTAATTGGGAACGACCAAATCTATA ACTCTATCGTCACAGCTCATGCTTTTATCATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGA TTAGTTCCTCTAATACTAGGAGCTCCAGATATAGCTTTCCCTCGTATAAATAATATAAGATTTTGATTACTTCCTCCTTC TATTTCATTACTATTAGCTAGAAGATTTATTGAGTCAGGGGCGGGAACAGGATGAACAGTTTACCCTCCTCTTTCCAGTA ATATTGCCCACAGTGGAGCCTCAGTAGATTTAACAATTTTTAGCCTTCATTTGGCAGGAATCTCTTCAATTTTGGGGGCA GTTAATTTTATCTCTTCAATTATAAATATACGAACCCCTGGAATAACAATAGAAAAAATACCTTTATTTGCTTGATCTGT AGGAATTACTGCCGTTCTATTACTTCTTTCTCTCCCAGTATTAGCAGGAGCAATTACAATACTTTTAACCGATCGAAATT TAAATACCTCATTTTTTGACCCAACCGGAnGAGGAGABLAST at The Wolbachia Project BLAST at NCBI
|Summary||The Insecta was found to be negative for Wolbachia.|