Japanese Burrowing Cricket (Velarifictorus micado)

November 16, 2023

Sample information
Picture
Photos by: Elijah S.
Location
Collection date 09/18/2023
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

This cricket is brown on top with a light beige underbelly. Long antennae. Little to differentiate torso from abdomen.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA EXTRACTION

It was hard to divide up the abdomen as it all kind of turned to goop, so we scooped up what we could and added it to the vial.

MISTAKES

We didn’t make any mistakes during the arthropod electrophoresis, but something went wrong for our wolbachia electrophoresis the first time we did it. Luckily, we repeated the experiment and all looks to have gone well.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We did make a mistake the first time we did our wolbachia electrophoresis, but we paid extra attention the next time, and everything looked to have gone perfectly well.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
AGTCGGCACATCACTAAGTATTTTAATTCGAACGGAATTAGGACAACCAGGGTATCTTATTGGAGATGATCAAACATATA ATGTAATTGTTACTGCACACGCCTTTATTATAATTTTCTTCATAGTAATACCAATTATGATTGGAGGATTCGGAAATTGA TTAGTTCCCTTAATATTAGGAGCACCCGATATAGCTTTCCCCCGAATAAATAATATAAGTTTTTGATTATTACCCCCTTC ATTAACTCTCCTATTAACCAGAAGAATAGTTGAAAATGGAGCAGGTACAGGATGAACAGTTTATCCACCCTTGTCAACAG GAATTGCCCATGCAGGAGCATCAGTTGATTTAGCAATTTTCTCATTACATCTCGCCGGAATCTCCTCCATTTTAGGTGCT GTAAATTTTATTACTACCATAATTAATATACGAGCTCCTAGAATGTCTTTAGATCAAACACCCCTATTTGTGTGAGCAGT AGGAATTACAGCACTATTATTATTATTATCATTACCTGTACTAGCAGGAGCTATTACTATACTATTAACTGATCGAAACT TAAATACCTCATTTTTTGATCCCGCAGGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
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