Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 11/13/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Arthropod was found under leaves inside of a small, shallow, slow-moving river in a tree-shaded area. |
| Putative identification | Mollusca Bivalvia Sphaeriida Sphaeriidae Pisidium |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | We did not see significant results after running gel electrophoresis; therefore, we do not know if the arthropod was wolbachia positive or not. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
CGGTTTGATGGGAACTGCTTTTAGAGTTATTGTTCGTATAGAATTAGCTTTACCTGGTTCATTGTTAAAAGATGCTCAGCTCTATAATGTTTTGGTTACTGCTCATGCTTTAGTAATGATTTTTTTTTTAGTTATACCAATAATAATGGGTGGATTTGGTAATTGATTGGTTCCGCTTATATTAGTGGTTCCTGATATGGCTTTTCCTCGTTTAAATA ATTTAAGATTTTGGTTATTACCTGTTGCATTATTATTGTTATTGGGTTCTGCATATGTGGAAAGGGGTGCTGGAACAGGATGAACTATTTATCCACCTTTATCTGGAAATGTTGCTCATGCTGGTCCTTCTATAGAATTTGCGATTTTATCTTTGCACTTAGGTGGTGCTTCATCTATTGCTGCATCAATTAATTTTTTAACTACTGTTTTAAACATA CGTTTAGGTGTTATAGAGTTAAAACGAGTGAGATTGTTTGTATTTTCTATTGCTATTACTGCTTTTCTATTAATTGTTGCAATACCTGTATTAGCTGGTGGTTTAACAATGCTTTTAACTGATCGAAACTTTAATACTACTTTTTTTGACCCAATAGGAAACGGGGATCCAGTTCTGTTTATACATATTTTTTGATTTTTTGGTCACCCTGGAAGTTTAA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
Water Boatman