Pill Bug

January 9, 2024

Sample information
Picture
Photos by: Rylee C
Location
Collection date 09/20/2023
Captive / Cultivated? Wild-caught
Group Scarborough High School
Observations

Pill bug collected outside of a suburban high school in Scarborough Maine. It was found crawling out of taller grass, onto a concrete sidewalk.
Putative identification

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

There was air bubbles during the extraction process

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

My confidence level is medium due to the arthropod negative control testing positive for arthropod. Another reason for my lower confidence is that my Arthropod tested negative for arthropod DNA. I believe there was a cross contamination during the PCR process and DNA that got into the negative control became amplified.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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