Toxomerus occidentalis

January 9, 2024

Sample information
Picture
Location
Collection date 09/20/2023
Captive / Cultivated? Wild-caught
Group Scarborough High School
Observations

Found the specimen in a parking lot behind the El Rayo restaurant, near the high school. There were large bushy trees and weeds that had blossoms that were white. I captured it while it was resting on one of the blossoms. There was lots of trash around.
Putative identification Arthropoda Hexapoda Insecta Diptera Syrphidae Toxomerus Toxomerus occidentalis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

H20 got contaminated probably by a pipette tip

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Our H20 had a slight line which could show contamination within the agarose.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Toxomerus occidentalis was found to be negative for Wolbachia.
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