Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/26/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Scarborough High School |
| Observations | The ant was found crawling up a tree in the field outside of Scarborough High School. There were no other ants around, nor was there a spotted ant hill. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Lasius Lasius niger |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | Dirt had fallen into our agarose gel during our gel electrophoresis reaction which was testing for arthropod. All of our test results were weak or absent so we believe the dirt could have played a role in contamination of our experiment. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | While the garden ant did have a clear positive result for wolbachia, it did not test positive for arthropod. All of the arthropod results were either faint or completely. We believe our gel may have been punctured by the micropipette tips during dispersion. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lasius niger was found to be postive for Wolbachia. |
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