Wolbachia Project Data

January 9, 2024

Sample information
Picture
Photos by: Meghan R
Location
Collection date 09/26/2023
Captive / Cultivated? Wild-caught
Group Scarborough High School
Observations

The ant was found crawling up a tree in the field outside of Scarborough High School. There were no other ants around, nor was there a spotted ant hill.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Lasius Lasius niger

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

Dirt had fallen into our agarose gel during our gel electrophoresis reaction which was testing for arthropod. All of our test results were weak or absent so we believe the dirt could have played a role in contamination of our experiment.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

While the garden ant did have a clear positive result for wolbachia, it did not test positive for arthropod. All of the arthropod results were either faint or completely. We believe our gel may have been punctured by the micropipette tips during dispersion.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lasius niger was found to be postive for Wolbachia.
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