small ant

March 15, 2024

Sample information
Picture
Photos by: Zain K.
Location
Collection date 08/29/2023
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

On sidewalk near the grass
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Only 1 band appeared when running a Duplex reaction with both Arthropod and Wolbachia primer. All constants performed as expected.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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