Ant

March 15, 2024

Sample information
Picture
Location
Collection date 08/29/2023
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

I found this ant on a sidewalk on the school campus. I found it in the morning, it was sunny and about 70 degrees F outside. There were many other ants in the area.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of the controls worked and the DNA also tested positive for Arthropod DNA meaning the DNA was extracted correctly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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