Portland State University – Boisea rubrolineata

October 31, 2023

Sample information
Picture
Location
Collection date
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Hexapoda Insecta Hemiptera Rhopalidae Boisea Boisea rubrolineata

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Lane 5 shows a positive for COI, Lane 6 is a positive control for Wolbachia, Lane 7 is blank for Wolbachia in the insect.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Boisea rubrolineata was found to be negative for Wolbachia.
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