Portland State University – Tipulidae

Sample information

Collection date 10/09/2023
Captive / Cultivated? Wild-caught
Group Portland State University
Putative identification Arthropoda Hexapoda Insecta Diptera Tipulidae


Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes


Wolbachia presence No
Confidence level High
Explanation of confidence level

Lane 2 shows positive for COI, Lane 3 is a Wolbachia positive control, Lane 4 is blank for Wolbachia in the insect.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Tipulidae was found to be negative for Wolbachia.
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