Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/09/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Portland State University |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Tipulidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Lane 2 shows positive for COI, Lane 3 is a Wolbachia positive control, Lane 4 is blank for Wolbachia in the insect. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Tipulidae was found to be negative for Wolbachia. |
Water Boatman