Portland State University – Vespula

October 31, 2023

Sample information
Picture
Photos by: Gabriella J.
Location
Collection date 10/07/2023
Captive / Cultivated? Wild-caught
Group Portland State University
Observations

Found near drainage pipe in urban area
Putative identification Arthropoda Insecta Hymenoptera Vespidae Vespula

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Lane 14 is positive for COI, Lane 15 is a Wolbachia positive control, Lane 16 shows a positive band for Wolbachia in the insect.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Vespula was found to be postive for Wolbachia.
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