Portland State University – Phaneroptera nana

Sample information

Photos by: Jonah R.
Collection date 10/07/2023
Captive / Cultivated? Wild-caught
Group Portland State University

Found under a leaf in a grassy, forested suburban area

Putative identification Arthropoda Hexapoda Insecta Orthoptera Tettigoniidae Phaneroptera Phaneroptera nana


Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes


Wolbachia presence No
Confidence level High
Explanation of confidence level

Lane 14 is positive for COI, Lane 15 is a Wolbachia positive control, Lane 16 is blank for Wolbachia in the insect.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Phaneroptera nana was found to be negative for Wolbachia.
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