Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/07/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Portland State University |
| Observations | Found under a leaf in a grassy, forested suburban area |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera Tettigoniidae Phaneroptera Phaneroptera nana |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Lane 14 is positive for COI, Lane 15 is a Wolbachia positive control, Lane 16 is blank for Wolbachia in the insect. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Phaneroptera nana was found to be negative for Wolbachia. |
Water Boatman