Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/17/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | I found this stink bug on some leaves under a tree at approximately 4 pm. The weather was about 75 degrees at the time of collection. I collected the bug in a box and cut out holes, so that it could breathe. It remained in this box for one day (before dissection). |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All of our controls showed up as we expected. Moreover, my sample did not match the positive control. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
GGNNCAGCAATAAGATNNNNNNTNCNNNNTGNNTTNNGACAACCAGGNNNATTCATTGGAGATGANCAAATNNNNNNTGTNNNNNNNACCGNNNNNNCNTTTGTAANAATTTNNNNNNNNNNNNNNNNNNNNNNNNTTGGAGGNNNNNGAAACNGATNNNNNNNNTTAATNNNNGGAGCACCTGATATAGCATTTCCGCGAATAAATAATATAAGATTTTGATTATTACCACCATCATTAACCTTATTAATAGTAAGAAGATTAGCAGAATCAGGAGCCGGGACTGGATGAACAGTATATCCCCCCTTATCAAGTAATTTATCACATGGAGGAGCATCAGTTGACTTAGCAATTTTTTCCCTACATTTAGCAGGAGTAACATCAATTTTAGGAGCAGTAAATTTTATTTCAACAATTATTANTATACGACCAATAGGAATAACCCCTGAACGAATTCCACTATTCGTTTGATCTGTTGGTATTACTGCATTATTATTATTATTATCACTCCCAGTACTAGCAGGGGCTATTACTATANNATTAACTGATCGAAATTTCAATACATCATTCTTTGACCCATCAGGAGGAGGAGATCCGATTCNTTNTCAACACTNNTTTTTGAT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hemiptera was found to be negative for Wolbachia. |
Water Boatman