|Location||No map location data available.|
|Captive / Cultivated?||Wild-caught|
|Group||Georgia Southern University|
Specimen was collected using a sweep net in a area with tall and unkept grass.
|Putative identification||Arthropoda Hexapoda Insecta Diptera|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
DNA extraction kit of in-house reagents was used.
|Explanation of confidence level||
The results of my controls (+,-,NTC) were all as expected. My positive control was positive for both the insect and Wolbachia gene. My negative control was positive for the insect gene and negative for the Wolbachia gene. My NTC was negetive for both genes. On top of that, my specimen tested positive for the insect gene. Becuase everything was as expected, I have high confidence in how the experiment was completed and therefore believe that my insect was not infected with Wolbachia.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Diptera was found to be negative for Wolbachia.|