BroACC_F23_MPT Diptera

November 6, 2023

Sample information
Picture
Photos by: McKenzie T
Location No map location data available.
Collection date 08/29/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Specimen was collected using a sweep net in a area with tall and unkept grass.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The results of my controls (+,-,NTC) were all as expected. My positive control was positive for both the insect and Wolbachia gene. My negative control was positive for the insect gene and negative for the Wolbachia gene. My NTC was negetive for both genes. On top of that, my specimen tested positive for the insect gene. Becuase everything was as expected, I have high confidence in how the experiment was completed and therefore believe that my insect was not infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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