GREACBF23-1 Hemiptera

November 6, 2023

Sample information
Picture
Location
Collection date
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Dry area under a tree in moss or semi-tall like grass.
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

Based off of local data which was very low I thought my bug would not have Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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