akf__Hemiptera

November 6, 2023

Sample information
Picture
Photos by: Alexis F.
Location No map location data available.
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Grassy field, in a bush, it was raining during the catch, used a net
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Looking at the Control groups and comparing results from PCR, the detected Wolbachia bacteria were there. I detected the Insect CO1 gene and the Wolbachia gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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