BroACC_F23_SOB

November 6, 2023

Sample information
Picture
Photos by: Shaylen B.
Location
Collection date 08/21/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Beat Stick and canvas
  • Garden in science center parking lot (Armstrong parking lot)
  • Put net under variety of bushes and plants and hit the plants
  • Time-1:40 pm
  • Weather-Overcast/light rain
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident that my insect did not have Wolbachia because there was no contamination and our data also had only one band at the insect gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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