November 6, 2023

Sample information
Picture
Photos by: Lauren n.
Location
Collection date 08/21/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

used a sweep net, Georgia southern Armstrong campus, bushes behind the garden
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

based on other data, prevalence is low in this insect order
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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