Stag Beetle

November 7, 2023

Sample information
Picture
Photos by: Evan M.
Location
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations

Arthropod was found under a rock that was held in place by a log from a tree.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

The DNA Ladder did not show up on the gels.

 
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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