Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/01/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | John Overton High School |
| Observations |
|
| Putative identification | Arthropoda Myriapoda Chilopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | My confidence level is low because the gel electrophoresis lab was inconclusive, the bands for arthropod did not show up or the band for Wolbachia would show up but not the band for arthropod so part of the DNA extraction or the PCR lab was invalid. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata
Cricket- Acheta domesticus