LOGACEF23-ANB HYMENOPTERA

November 7, 2023

Sample information
Picture
Photos by: Ariyana B
Location
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

We use a sweeping net and walked out to the back area of the science building parking lot on Armstrong Campus at 9:51am. It was located on a curb in the grass. It was 84 degrees outside, sunny but partly cloudy. We used the sweeping net to first place on top of the insect then did a swinging motion the air to keep it inside the net.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

Somewhere along the experimental process, the positive control was got messed up making the results inconclusive.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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