Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 08/21/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collection Method: Searching the ground and picking up with forceps. |
| Putative identification | Arthropoda Insecta Dermaptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | My results were inconclusive, so I’m not very sure if it was positive or negative for Wolbachia. |
| Wolbachia 16S sequence |
ANGTATTCNCNNGNNGTGCTGATCCNNATTACTANCGATTCCAANTTCNNGNCTCGAGTTGCAGAGT ACAATCCGAACTGAGATGGNTTTTAAGGGATTANCTTANCCTCGCGACTTTGCAGCCCATTGTANCC ACCATTGTANCACGTGTGTANCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCC TCCAGTTTATCACNGGCAGTTTCCTTANNGTCCCCAGCATTACCTGATGGTAANNAAGGATGAGGGT TGCGCTCGTTGCGGGACTTAACCCANCATCTCNCGACACGAGCTGACGACAGCCCTGCNACACCTG TGTGAANCCCGGNNNANNCNACCCTATCCCTTCNAATANGTNTGA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
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| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid