LOGACGF23-JM- Dermaptera

November 7, 2023

Sample information
Picture
Photos by: Ja'Bree H
Location No map location data available.
Collection date 08/21/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collection Method: Searching the ground and picking up with forceps.
Putative identification Arthropoda Hexapoda Insecta Dermaptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

My results were inconclusive, so I’m not very sure if it was positive or negative for Wolbachia.
Wolbachia 16S sequence
ANGTATTCNCNNGNNGTGCTGATCCNNATTACTANCGATTCCAANTTCNNGNCTCGAGTTGCAGAGT ACAATCCGAACTGAGATGGNTTTTAAGGGATTANCTTANCCTCGCGACTTTGCAGCCCATTGTANCC ACCATTGTANCACGTGTGTANCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCC TCCAGTTTATCACNGGCAGTTTCCTTANNGTCCCCAGCATTACCTGATGGTAANNAAGGATGAGGGT TGCGCTCGTTGCGGGACTTAACCCANCATCTCNCGACACGAGCTGACGACAGCCCTGCNACACCTG TGTGAANCCCGGNNNANNCNACCCTATCCCTTCNAATANGTNTGA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary
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