Horned Passalus Beetle-Odontitaenius disjunctus

November 7, 2023

Sample information
Picture
Photos by: Parker N.
Location
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations

Found under a rotting log in the woods. Beetle is black with straight line indents along the back. The beetle also has antennas, pinchers, and six legs.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location body without wings
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no bar at all around 438bp which is where a positive Wolbachia Gene would be located. However, my sample contained the Arthropod Barcoding Gene (CO1).
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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