BROACH_F23ACP_Hempitera

November 8, 2023

Sample information
Picture
Photos by: Amya Phillips
Location No map location data available.
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Tall grassy area surrounded by trees
  • Sweep net (Catch bugs),
  • Collection Jar (Store bugs), Forceps (Picks up bugs)
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My results were negative
NTC was not contaminated
positive showed two bands
negative control showed only one band
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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