Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/23/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collection Method: Sweeping Vegetation using a Net Environment: GSU Vegetation, 86ยบ F, Windy and Partly Cloudy |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The gel clearly indicates a bar in line with the + Control for the specified Wolbachia 16S rRNA gene for specimen MACACIF23-10. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be postive for Wolbachia. |
Water Boatman