BROH-F23BLL, ORTHOPTERA

Sample information

Picture
Photos by: BREANNA L.
Location No map location data available.
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Tall, grassy area

Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods

Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results

Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The results for the PCR come out correct and there were no contamination.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be postive for Wolbachia.
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