BroACH_F23JPL Orthoptera

November 8, 2023

Sample information
Picture
Photos by: Johana Pierre Louis
Location No map location data available.
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • from 1:30-2pm @ GSU, Armstrong Campus
  • under trees, grass, & ground (near parking lot)
  • Canvas catching net method, forceps method, mat and beat stick method

 
Putative identification Arthropoda Hexapoda

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

For the + control, the insect COI gene and Wolbachia gene were +

For – control, insect gene and Wolbachia gene were –

For NTC, the insect gene and Wolbachia gene were –

I determined the insect gene
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
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