MAC.AIC.F23-17

November 8, 2023

Sample information
Picture
Photos by: Ali R.
Location No map location data available.
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

I collected my insect with a net outside the Georgia Southern Armstrong Science Center. It was 1 p.m., sunny, and 87 degrees.
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I highly do not believe there is any Wolbachia in my insect. I believe this because local data has shown only an 8% chance of a Lepidoptera containing Wolbachia. The gel electrophoresis also produced a negative result for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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