Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 08/23/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | I collected my insect with a net outside the Georgia Southern Armstrong Science Center. It was 1 p.m., sunny, and 87 degrees. |
| Putative identification | Arthropoda Hexapoda Insecta Lepidoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I highly do not believe there is any Wolbachia in my insect. I believe this because local data has shown only an 8% chance of a Lepidoptera containing Wolbachia. The gel electrophoresis also produced a negative result for Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lepidoptera was found to be negative for Wolbachia. |
Water Boatman