LOGACEF23-ST Hemiptera

November 9, 2023

Sample information
Picture
Photos by: Samantha T
Location No map location data available.
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am very confident that my insect is negative Wolbachia. We had no issues with any controls, and did everything the proper way. I am confident based off the gel electrophoresis results, that my insect is negative with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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