Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 11/01/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | John Overton High School |
| Observations | This insect was found at Long Hunter State Park on a cold day with a high of 51 degrees. It was found towards the beginning of the trail in a small swamp, and this swamp was filled with these hexapoda insects, jumping across the water. |
| Putative identification | Arthropoda Hexapoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | The gel electrophoresis results showed no bands for our samples. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata
Cricket- Acheta domesticus