E3

Sample information

Picture
Location
Collection date 10/14/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations
Putative identification Arthropoda Hexapoda Insecta Diptera Simuliidae

Methods

Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

Controls worked, clearly had no band, and appeared accordingly.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Simuliidae was found to be negative for Wolbachia.
Report Inappropriate Post