Sample information
Picture
Location
Collection date	10/14/2022
Captive / Cultivated?	Wild-caught
Group	College of Eastern Idaho
Observations
Putative identification	Arthropoda Hexapoda Insecta Diptera Simuliidae
Methods
Extraction kit	Edwards Buffer
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	Controls worked, clearly had no band, and appeared accordingly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Simuliidae was found to be negative for Wolbachia.