Sample information
Picture
Location
Collection date	10/14/2022
Captive / Cultivated?
Group	College of Eastern Idaho
Observations
Putative identification	Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster
Methods
Extraction kit	Edwards Buffer
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	Medium
Explanation of confidence level	Controls worked, had a band, but it was faint. Did come back from sequencing.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Drosophila melanogaster was found to be postive for Wolbachia.