Odorous house ant (Tapinoma sessile)

November 20, 2023

Sample information
Picture
Photos by: Landry D
Location
Collection date 09/14/2023
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

Caught in the sun on concrete
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Controls worked as expected and results showed that no Wolbachia was present
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
CCTTTTATAATnnnnnnnnnnnnnnnnnnnnnnNNNnnnnnNnnnnnCnnnnnnnnAGATATAGCTTACCCnCnAATAAATAATATAAGATTTTGATTAnTACCCCCCTCAnnnnnnnnnnnAACnAnnnnAAnnTTnnnnAGATCnGGnGTAnnnnnnnnNnnAACAnTTTACCCnCCnCnAGCATCTAATAnnnnnCATAATGGACCTTCnGTAnAnTTAGCTATTTTnnCnCTTCATATTGCnGGTATATCnTCAATTTTAGGGGCAATTAATTTTATTTCTACTATTATTAATATnnnnnnTAAAAATTTTnCNnCnGATAAnnnnnnTCnnnnAnTnnnATCnnnnnnAATTACTGCAATTTTATTN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formicidae was found to be negative for Wolbachia.
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