Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/10/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Fall, Urban, hot temp Brown, 6 legs with hairs on the back pair. Semi translucent shell, 2 antennae, armored head. |
| Putative identification | Arthropoda Hexapoda Insecta Blattodea |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | There was no band for Wolbachia under E3, and there was a strong band under the positive control. Researchers at Penn State sent DNA sequences that matched the visual identification. |
| Wolbachia 16S sequence | Download AB1
|
| Arthropod COI sequence | Download AB1
|
| Summary | The Blattodea was found to be negative for Wolbachia. |
Sehirus cinctus
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus