Amynthas Carnosus

November 14, 2023

Sample information
Picture
Photos by: Sebi H
Location
Collection date 09/13/2023
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

The worm was dying, and a group of ants were swarming around it and trying to eat it.
Putative identification Annelida Clitellata Opisthopora Megascolecidae Amynthas Amynthas carnosus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no band at all for my sample in the Wolbachia gel.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GGTATGAGACTACTTATTCGAATCGAGCTCANGCAACCGGGCTCCTTTCTGGGAAGGGACCAACTATATA ACACANTTGTAACAGCACACGCATTCCTTATAATTTTCTTTCNAGTTATACNNGTATTCATTGGGGGATT TGGAAATTGGCTACTACCACTCATACTGGGAACACCGGACATAGCATTTCCACGCCTAAACAACATGAGA TTCTGGCTACTGCCCCCATCACTAATCTTACTAGTATCTTCCGCCGCANTANAGAAAGGAGCAGGTACAG GATGAACAGTATATCCCCCCCTANCAAGAAATATTGCTCATGCGGGGCCATCAGTAGACCTGGCAATTTT CTCACTCCACTTANCTGGGGCATCNTCNNTTTTGGGGGCCATTAACTTTATTACTACAGTAATTAATANG CNCTGGTCTGGACTACGTCTAGAGCGAATCCCGCTATTTGTATGAGCAGTAGTAATTACTGTCGTACTAC TATTACTATCATTACCAGTTCTTGCCGGANCTATTACAATACTACTAACAGACCNAAACCTAAATACATC ATTCTTCGACCCNNCGGGANGGGGGGACCCAATTCTGTACCAACACCTATTTTGATTTTTTGGTCACCCT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Amynthas carnosus was found to be negative for Wolbachia.
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