Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/14/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | Nesting in a hole, aggressive upon attempted capture, has a stinger. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Vespidae Vespula Vespula maculifrons |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All of the controls worked and there was no band denoting Wolbachia presence in my gel. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
TTTAGGAGCTTCAATAAGAATAATTATTCGCTTAGAATTAAGATCTCCTGGAGCCTTAATTAATAATGATCAAATTTATAATACAATTATTACAGCTCATGCCTTTATTATAATTTTCTTTATAGTTATACCTTTTTTAGTAGGGGGATTTGGAAACTGATTAATCCCTTTAATACTAGGTGTACCTGATATAGCATTTCCTCGAATAAATAATATAAGATTTTGATTACTCCCTCCTTCATTATTTTTACTAATTTTAAGAAATTTTATTGGAACAGGGGTAGGAACAGGATGAACTTTATACCCTCCTTTATCTTCTATTGTTGGTCATGATTCTCCATCTGTAGATTTAGGAATTTTTTCAATTCATATTGCTGGAATTTCATCAATTATAGGATCAATTAATTTTATCGTTACTATTTTAAATATACACACAAAAACACATTCATTAAATTTTCTTCCTTTATTCACATGATCAATTTTAATTACAGCAATTCTTCTTCTATTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTTTTAACAGATCGGAACTTAAATACATCTTTTTTCGATCCTGCAGGTGGGGGGGANCCAATTTTATAT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Vespula maculifrons was found to be negative for Wolbachia. |
Joro Spider
Imported Red Fire Ant
Black carpenter ant
Flocculent tailed millipede
Tawny crazy ant