Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 11/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | found near a tree, orange, very small, found alone Our initial ID was Wasmannia auropunctata, but our BLAST showed it to be Prenolepis impairs |
| Putative identification | Arthropoda |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | The gel results for Wolbachia did not run as we expected, so we cannot conclude if it has Wolbachia or not. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
>ZS_3_Wasmannia-CO1F.ab1 (601 bp) TCAATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATAGT TACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTT TAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTA CTTTTATTAAGAAATTTCATTAATGACGGAGTGGGAACAGGATGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCA TAATGGTCCTTCAGTTGACCTGACAATTTTTTCTCTACATATTGCAGGAATATCTTCTATTCTAGGAGCTATCAATTTTA TTTCAACAATTCTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTACA GCAATTTTACTTCTTCTATCTTTACCAGTTCTAGCTGGAGCTATTACTATACTTTTAACTGACCGGAACCTTAATACTTC CTTTTTTGACCCCTCAGGAGGAGGAGACCCTGTTCTTTATC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
Metriocnemus fuscipes YC1
Trachelipus ratzeburgii YC2
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1