Sample information |
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| Picture |
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| Location | |
| Collection date | 11/13/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Found under a log. Contains multiple legs. Red spots on it, brown colored body. 2 cm long. No antennae. 40 legs. 19 segments. White legs. *Initial identification is Euryurus leachii, however after Blast, the identification is Euryrurs orestes. |
| Putative identification | Arthropoda Myriapoda Diplopoda Polydesmida Euryuridae Euryurus Euryurus leachii |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | Cut in the 3rd section of the Euryurys leachii to obtain the reproductive organs. Gel electrophoresis did not work as well as predicted. |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | The gel for Wolbachia did not run, therefore it is inconclusive if the arthropod has Wolbachia or not. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
>ZS_1_Euryuvus-CO1F.ab1 (602 bp) CTCTGCTTTGAGAAAATAATTCGTTTAGAGCTTGGGCATTCTGGGAGATTGATTGGGGATGATCAGATTT ATAATGTAATTGTTACTGCACATGCTTTTGTTATAATTTTTTTTATGGTGATACCTATTATAATTGGGGG GTTTGGTAATTGATTGGTCCCTATTATAATTGGGGCTCCTGATATGGCTTTTCCCCGAATGAATAATTTA AGTTTTTGATTGTTGCCCCCCTCTTTTTTTTTATTATTAGCTTCTTCTTTGGTTGAAAGGGGGGTTGGGA CTGGGTGAACTGTATATCCACCGTTGGCAGCTAGACTTTTTCATGGGGGCCCAGCTGTGGATTTAGCTAT TTTTTCTTTACATTTGGCTGGGGCATCATCTATTTTGGGTGCTATTAATTTTATTACTACTGTTATTAAT ATGCGAACTTATGGAATGGTATTTGAGCGGATGCCTTTATTTGTTTGATCTGTTGTTATTACTGCTGTTT TATTATTACTTTCTCTTCCTGTGTTGGCTGGGGCTATTACTATATTATTAACTGATCGGAATTTTAATAC TACTTTTTTTGATCCTGCCGGGGGCGGGGATCCTATTTTATA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
Water Boatman