Euryrus orestes

April 26, 2024

Sample information
Picture
Location
Collection date 11/13/2023
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Found under a log. Contains multiple legs. Red spots on it, brown colored body. 2 cm long. No antennae. 40 legs. 19 segments. White legs.

*Initial identification is Euryurus leachii, however after Blast, the identification is Euryrurs orestes. 
Putative identification Arthropoda Myriapoda Diplopoda Polydesmida Euryuridae Euryurus Euryurus leachii

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Cut in the 3rd section of the Euryurys leachii to obtain the reproductive organs. Gel electrophoresis did not work as well as predicted.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

The gel for Wolbachia did not run, therefore it is inconclusive if the arthropod has Wolbachia or not.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
>ZS_1_Euryuvus-CO1F.ab1 (602 bp) CTCTGCTTTGAGAAAATAATTCGTTTAGAGCTTGGGCATTCTGGGAGATTGATTGGGGATGATCAGATTT ATAATGTAATTGTTACTGCACATGCTTTTGTTATAATTTTTTTTATGGTGATACCTATTATAATTGGGGG GTTTGGTAATTGATTGGTCCCTATTATAATTGGGGCTCCTGATATGGCTTTTCCCCGAATGAATAATTTA AGTTTTTGATTGTTGCCCCCCTCTTTTTTTTTATTATTAGCTTCTTCTTTGGTTGAAAGGGGGGTTGGGA CTGGGTGAACTGTATATCCACCGTTGGCAGCTAGACTTTTTCATGGGGGCCCAGCTGTGGATTTAGCTAT TTTTTCTTTACATTTGGCTGGGGCATCATCTATTTTGGGTGCTATTAATTTTATTACTACTGTTATTAAT ATGCGAACTTATGGAATGGTATTTGAGCGGATGCCTTTATTTGTTTGATCTGTTGTTATTACTGCTGTTT TATTATTACTTTCTCTTCCTGTGTTGGCTGGGGCTATTACTATATTATTAACTGATCGGAATTTTAATAC TACTTTTTTTGATCCTGCCGGGGGCGGGGATCCTATTTTATA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
Report Inappropriate Post