Formica subsericea

November 17, 2023

Sample information
Picture
Location
Collection date 11/14/2023
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Under larger rocks, dry leaves. Black in color, antennae, 3 segments in body, 0.9 cm.

*Initial ID was Lasius niger, however after blast, we concluded that the arthropod was Formicia subsericea. 
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Lasius Lasius niger

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Extracted reproductive organs from body. Ran gel.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

The Wolbachia gel did not run properly so we are unable to provide conclusive results.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
>ZS_2_Lasius-CO1F.ab1 (601 bp) TTCTATAAGTATAATTATTCGTTTAGAATTAGGTTCATCTAATTCATTAATCAATAATGATCAAATTTAT AATTCTTTAGTAACTAATCACGCTTTTATTATAATTTTTTTCATAGTAATACCATTTATAATTGGTGGGT TTGGTAATTTTCTAATTCCTTTAATACTAGGATCACCAGACATAGCATACCCTCGTATAAATAATATAAG ATTTTGACTCTTACCTCCTTCAATTACTCTTTTACTTTTAAGAAATTTTATTAATGATGGAACAGGAACA GGATGGACTATTTATCCTCCCTTATCCTCTAATATTTTTCATAGTGGTCCTTCTGTAGACTTAACAATTT TTTCTCTTCATATTGCAGGTATATCCTCAATTTTAGGAGCAATTAATTTTATTTCAACAATTCTTAATAT ACATCATAAAAATTTTTCTATTGATAAAATTCCTTTACTCGTATGATCAATTTTAATTACAGCTATCTTA CTTCTATTATCCTTACCTGTATTGGCCGGAGCTATTACTATACTATTAACTGATCGAAATTTAAATACTT CATTCTTTGATCCTTCGGGAGGAGGCGATCCAATTCTATAT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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