Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/14/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Under larger rocks, dry leaves. Black in color, antennae, 3 segments in body, 0.9 cm. *Initial ID was Lasius niger, however after blast, we concluded that the arthropod was Formicia subsericea. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Lasius Lasius niger |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Extracted reproductive organs from body. Ran gel. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | The Wolbachia gel did not run properly so we are unable to provide conclusive results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
>ZS_2_Lasius-CO1F.ab1 (601 bp) TTCTATAAGTATAATTATTCGTTTAGAATTAGGTTCATCTAATTCATTAATCAATAATGATCAAATTTAT AATTCTTTAGTAACTAATCACGCTTTTATTATAATTTTTTTCATAGTAATACCATTTATAATTGGTGGGT TTGGTAATTTTCTAATTCCTTTAATACTAGGATCACCAGACATAGCATACCCTCGTATAAATAATATAAG ATTTTGACTCTTACCTCCTTCAATTACTCTTTTACTTTTAAGAAATTTTATTAATGATGGAACAGGAACA GGATGGACTATTTATCCTCCCTTATCCTCTAATATTTTTCATAGTGGTCCTTCTGTAGACTTAACAATTT TTTCTCTTCATATTGCAGGTATATCCTCAATTTTAGGAGCAATTAATTTTATTTCAACAATTCTTAATAT ACATCATAAAAATTTTTCTATTGATAAAATTCCTTTACTCGTATGATCAATTTTAATTACAGCTATCTTA CTTCTATTATCCTTACCTGTATTGGCCGGAGCTATTACTATACTATTAACTGATCGAAATTTAAATACTT CATTCTTTGATCCTTCGGGAGGAGGCGATCCAATTCTATAT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | |
Water Boatman