Pharaoh Ant – Riya

Sample information

Photos by: Riya D.
Collection date 11/13/2023
Captive / Cultivated? Wild-caught
Group Walton High School

The ant is an orange brown color and about 2 mm long. It has 3 distinct segments as well as a head with 2 large compound eyes. It was found near a wooden bench in some dry dirt buried under leaf litter. It was very squirmy and wanted to leave the test vial.

Putative identification Arthropoda


Extraction kit
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system
DNA stain
Gel images
Protocol notes


Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Most of the colony I found the ant from was probably worker ants, so their development stages were still very low. In this case, Wolbachia will probably die in the organism before it can be passed on

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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