Tenuiphantes zebra

April 26, 2024

Sample information
Picture
Photos by: Allison Ray
Location
Collection date 11/13/2023
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Spider is small and reddish brown. Legs are a light brown, almost yellow, color. The abdomen has white dots along the sides and is covered in short, fine hairs.
Putative identification Arthropoda Chelicerata Arachnida Araneae Linyphiidae Tenuiphantes Tenuiphantes zebra

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The reproductive organs were undoubtedly captured in the extraction process because the entire organism was used. Because the positive and negative controls all worked, it is likely that there were no errors, and the negative result on the gel is correct.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
>Tenuiphantes zebra AACTGCGATAAGAGTATTAATTCGAATTGAATTAGGACAAACTGGGAGAATATTGGGAGATGACCAATTA TATAATGTGATTGTTACTGCACATGCTTTTGTTATAATTTTTTTTATGGTTATACCAATTTTAATTGGGG GGTTTGGAAATTGGTTAGTTCCTTTAATGCTTGGGGCTCCTGATATAGCTTTTCCACGGATAAATAATTT AAGATTTTGGTTATTACCCCCTTCTTTGTTATTGTTATTTATTTCAAGAATGGTTGAAATAGGAGTTGGG GCGGGATGAACAGTGTATCCCCCTCTTGCTTCTTTGGAAGGTCATGCTGGGAGTTCTGTTGATTTTGCTA TTTTTTCTTTACATTTGGCTGGGGCATCTTCAATTATAGGAGCTATTAATTTTATTTCTACTATTGTTAA TATACGAGGGTGTGGTGTATCGATAGAGAGGGTCCCTTTATTTGTGTGATCTGTATTAATTACTGCTGTT TTGTTATTGTTATCATTACCTGTGTTAGCAGGTGCTATCACAATGTTGTTAACTGATCGAAATTTTAATA CTTCCTTTTTTGACCCTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Tenuiphantes zebra was found to be negative for Wolbachia.
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