Tigerosa helluo

Sample information

Photos by: Vikram M
Collection date 11/13/2023
Captive / Cultivated? Wild-caught
Group Walton High School

A juvenile wolf spider found scavenging among leaf litter about 3 meters perpendicular to a stream.

Putative identification Arthropoda


Extraction kit DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes


Wolbachia presence No
Confidence level Low
Explanation of confidence level

We did not have any distinct bands; a possible cause of this is that we did not get a chance to see the fresh PCR product and had to wait about a day, which could have resulted in diffusion. Another is that there was an error in amplification or set up, resulting in a failure of replication, in which case the bands seen are just the stain, or possibly non-specific DNA products. This organism was the second column from the right.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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