Tigerosa helluo

Sample information
Picture	Photos by: Vikram M
Location
Collection date	11/13/2023
Captive / Cultivated?	Wild-caught
Group	Walton High School
Observations	A juvenile wolf spider found scavenging among leaf litter about 3 meters perpendicular to a stream.
Putative identification	Arthropoda
Methods
Extraction kit	DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level	We did not have any distinct bands; a possible cause of this is that we did not get a chance to see the fresh PCR product and had to wait about a day, which could have resulted in diffusion. Another is that there was an error in amplification or set up, resulting in a failure of replication, in which case the bands seen are just the stain, or possibly non-specific DNA products. This organism was the second column from the right.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Arthropoda was found to be negative for Wolbachia.

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