Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/19/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | This arthropod was found in a science classroom of the Saint Albans School in Washington, DC in early September. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Curculionidae Pseudoedophrys Pseudoedophrys hilleri |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | Analysis: We had to rerun the arthropod DNA amplification for a second time to get the DNA to appear on a gel. The uploaded photo is from the second try, which turned out to be successful. In our first attempt, the DNA of the arthropod controls and of one other specimen were successfully amplified, but the DNA control and my specimen were not. We then reran PCR, but using a slightly larger quantity of DNA than we had the first time. This rerun produced the expected results.
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Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Our controls all worked as expected, so we have no reason to doubt the outcome of gel electrophoresis. Additionally, another bug on the gel did test positive for Wolbachia, reenforcing that the gel was run properly. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
GAAATTGACTANTACCTTTAATATTAGGAGCCCCAGATATAGCCTTTCCTCGACTAAATAATATAANATTTTGGCTTCTCCCTCCTTCTCTCTCCCTTCTATTANTAANAANAATTGTGGATAAAGGAGCAGGGACAGGATGAACCGTATACCCGCCCNTATCTGCTAATATCGCTCACGAAGGCTCATCTGTANACCTAGNTNTTTTCNNACTACATATAGCAGGAATTTCATCANTCCTANGAGNNNNNNNTTTTATTTCTACGGTAATTAATATACGTCCTTCANGAATATCATTTGATCGTTTACCTCTTTTTGTATGANCCGTTAAAATTACTGCTA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Pseudoedophrys hilleri was found to be negative for Wolbachia. |
Water Boatman